Interaction of Pyridoxamine-Pyruvate Transaminase with Carbonyl Derivatives of Pyridoxal
نویسندگان
چکیده
منابع مشابه
Interaction of Pyridoxamine-pyruvate Transaminase with Carbonyl Derivatives of Pyridoxal.
on pyridoxine or pyridoxamine as a sole source of carbon and nitrogen (1, 2). This transaminase contains no pyridoxal phosphate, and hence provides a simpler system for mechanistic studies than do the more complex, pyridoxal phosphate-containing transaminases. The enzyme binds pyridoxamine and pyridoxal with almost equal avidity; the latter is bound in part by an azomethine Iinkage to the e-ami...
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The enzyme-substrate complex formed between pyridoxamine-pyruvate transaminase (EC 2.6.1.30) and pyridoxal was reduced with NaBH4. After carboxymethylation and tryptic digestion, pyridoxyl-lysine-containing peptides were isolated by a combination of Sephadex and Dowex 50 chromatography. Analysis of these peptides shows the structure around the pyridoxal-binding lysine residues to be Ala-Asp-Ile...
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Wherever the point has been examined (e.g. References l-6), pyridoxal phosphate enzymes have been found to require free sulfhydryl groups for activity. The functional role played by these groups, however, has not been clear. In glutamate-oxaloacetate transaminase, which has been studied most extensively, no spectrophotometric or enzymatic evidence for their participation in coenzyme binding was...
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برهمکنش dnaتیموس گاوی طبیعی (ct-dna) با اتیلن دی آمین تترااستات (edta)در بافرtris-hcl با 8/7 ph ( دراین ph،edta به نمک دی سدیم تبدیل می شود) وسسامول در بافر tris-hcl با4/7 ph مورد بررسی قرار گرفته است. edta و سسامول استفاده فراوانی در تکنولوژی غذایی و صنعت شیمیایی دارند. مدل اتصال dna مربوط بهedta بوسیله اسپکتروفتومتری جذب، دورنگ نمایی حلقوی(cd)، ویسکومتری وژل الکتروفورز بررسی شده است. طیفuv ...
15 صفحه اولMolecular cloning, expression and characterization of pyridoxamine-pyruvate aminotransferase.
Pyridoxamine-pyruvate aminotransferase is a PLP (pyridoxal 5'-phosphate) (a coenzyme form of vitamin B6)-independent aminotransferase which catalyses a reversible transamination reaction between pyridoxamine and pyruvate to form pyridoxal and L-alanine. The gene encoding the enzyme has been identified, cloned and overexpressed for the first time. The mlr6806 gene on the chromosome of a symbioti...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1965
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)97284-4